The PF-3758309 resistant CRC cell line, HT29, was transfected with shRNA for E-cadherin, whereas the PF-3758309 sensitive CRC cell line, RKO, was transfected with shRNAs against vimentin, caldesmon, Zeb-1, or the miRNA 200c expression vector (miRNA 200c suppresses Zeb-1 expression). The gene discussed is CALD1; the disease is colorectal carcinoma.