SQSTM1 and hepatocellular carcinoma: Using the iTRAQ-2DLC-ESI-MS/MS technique, we recently identified 147 proteins, including 52 that were up-regulated and 95 that were down-regulated in small HCC, and identified aminoacylase-1 (ACY1) and sequestosome-1 (SQSTM1) as candidate immunohistochemical markers for distinguishing between small HCCs (<3 cm) and DN [15].