GPC3 and hepatocellular carcinoma: In the present study, we therefore analyzed the expression patterns of ACY1, SQSTM1, and GPC3 among low-grade DN (LGDN), HGDN, WDHCC and moderately differentiated HCC (MDHCC), and determined the accuracies of different panels of markers using ACY1, SQSTM1, and GPC3.