In particular we used CCRF-CEM cell line as reference for KRAS mutation p.G12D (heterozygous), the human colorectal carcinoma cell line SW620 (used as reference for KRAS mutation pG12V, homozygous), and the human melanoma cell lines A375 (used as the source of homozygous BRAF V600E DNA). This evidence concerns the gene BRAF and melanoma.