SRC and breast cancer: To study the role of the Src kinase protein on mechanotransduction and motility, we generated stable MDA-MB-231 breast cancer cell clones with similar expression of GFP-tagged wild type Src protein (GFP-wt-Src, W2E9 clone) and a GFP-tagged mutant of Src protein, c-Src(Y527F), rendering it constitutively active (GFP-ca-Src, C1G1 and C2E8 clones) (Fig. 1, α-Src and α-GFP).