As expected, the phosphorylation of AKT, GSK-3β, PRAS40, S6K and 4E-BP1 was markedly reduced by NVP-BEZ235 but the total proteins of them were not affected in CNE2 and HONE1cells (Fig. 2), which was consistent with studies of other tumors, such as breast cancer, gliomas and myeloma [12], [21], [22]. The gene discussed is GSK3B; the disease is breast cancer.