Purified CD4+ T cells were stimulated with beads coated with anti-CD3 and anti-CD28 antibodies and co-transduced with a lentivirus expressing codon-optimized CD8α and individual CD8β isoforms and a second lentivirus expressing TCRαβ from a CD8 dependent TCR recognizing the melanoma antigen NY-ESO-1 (Figure 6A) [34]. Here, CD8B is linked to melanoma.