In summary, our imaging demonstrates a loss of lung CD11c+CD103+ DCs that corresponded to an influx of highly active DCs into the mediastinal lymph node on day 1 after virus infection, consistent with previous reports of rapid exit of lung-resident CD11c+CD11b−CD103+ DCs followed by arrival in the draining lymph node post-influenza infection [16], [26]. This evidence concerns the gene ITGAX and influenza.