Four cell lines were selected for further in vitro and in vivo analyses: SY5Y (single copy-MYCN) and KCNR (MYCN-amplified) from NB which were in group that was most sensitive to AZD1480; Rh18 (RMS) which was in the group showing intermediate sensitivity to AZD1480 and TC32 (ESFT) which was in the group showing the least sensitivity to AZD1480. The gene discussed is MYCN; the disease is neuroblastoma.