CHAT and amyotrophic lateral sclerosis: Finally, considering that Tdp-43, also linked to ALS etiopathogenesis, is involved in multiple steps of RNA metabolism, including transcription, splicing, or transport of mRNA (Lagier-Tourenne and Cleveland 2010), as well as microRNA metabolism, and it has been recently shown to target ChAT mRNA as well, (Buratti et al. 2010) we wanted to analyze its expression at early presymptomatic stages in this mouse model.