In addition, either short-term or life-long CR protected the mouse heart against ischemia-reperfusion damage in the Langendorff model, and the cardioprotection was abolished by the AMPK inhibitor adenine 9-β-D-arabinofuranoside (AraA) that was given intravenously [21] or added to the perfusate before the onset of ischemia [43]. Here, PRKAA2 is linked to ischemia.