To determine whether PKR is required for 2-ME-mediated autophagic flux, we have investigated the effect of 2-ME on autophagosome formation and LC3-I to LC3-II conversion, in osteosarcoma cells that have been stably-transfected with trans- dominant mutant PKR cDNAs and are defective for PKR activity and resistant to 2-ME-mediated anti-proliferative effects. Here, EIF2AK2 is linked to osteosarcoma.