CCR5 and HIV-1 infection: To investigate the role of IL-27 in HIV-1 infection of DCs, iDCs and mDCs were infected with the CCR5 tropic virus HIV-1Ba-L, the cells were cultured in the presence or absence of 100 ng/ml of IL-27 and p24 levels were measured after 14 days to assess levels of cis HIV-1 infection (Figure 3A).