FN1 and breast carcinoma: Using the 3D PEGDA matrix with a certain stiffness, we demonstrated that the cell adhesion CD44 binding peptide (CD44BP), RGD integrin binding peptide (IBP), and fibronectin-derived heparin binding peptide (FHBP) can be individually conjugated to the inert PEGDA gel and their effect on the maintenance of breast cancer stem cells can be investigated without the interference of other factors.