CD4 and neoplasm: In contrast, we found little or no difference in IL-2 or IFN-γ production between Tim-3+ and Tim-3− CD4 T cells derived from NILs, whereas circulating Tim-3+ CD4 T cells produced even more IFN-γ than Tim-3− cells (Figure 2A), suggesting that the tumor environment can profoundly affect the phenotype of Tim-3+ CD4 T cells.