To determine whether Tim-3+PD-1+ Tregs exist in human tumor tissues, we compared the expression of Foxp3 in Tim-3 single positive (Tim-3 SP), PD-1 single positive (PD-1 SP), Tim-3 and PD-1 double positive cells (DP) and Tim-3 and PD-1 double negative (DN) cells. The gene discussed is HAVCR2; the disease is neoplasm.