APOE and cardiovascular disorder: Aortic arch calcification has been shown to be independently associated with an increased risk of cardiovascular diseases.4 We previously demonstrated that apolipoprotein E (apoE)–null mice with a 129S6/SvEvTac strain background (129‐apoE KO) develop more atherosclerotic plaques in the aortic arch than those with a C57BL/6J background (B6‐apoE KO).5–7 In this study, we explored the feasibility of detecting calcification in aortic arch plaques using CNT‐based micro‐CT and compared aortic arch calcification volume between the 2 strains of apoE‐knockout (KO) mice.