The analysis of membrane-enriched extracts immunoprecipitated with anti-CD63 antibody followed by Western blot using anti-β1-integrin antibody showed that β1-integrin-CD63 interaction occurs in all lineages, but is more intense along melanoma progression (4C, 4C11- and 4C11+ cell lines) (Figure 2C). This evidence concerns the gene CD63 and melanoma.