Although timp1 transcript is increased in metastatic 4C11+ melanoma cell line compared to pre-malignant 4C and non-metastatic 4C11- melanoma cell line (Figure 1A) and Timp1 protein is augmented at cell surface since pre-malignant 4C to metastatic 4C11+ cell line (Figure 1B), its presence in the supernatant is more expressive in pre-malignant 4C and non-metastatic 4C11- cells compared to melan-a melanocytes (Figure 1C). This evidence concerns the gene TIMP1 and melanoma.