CEACAM5 and colorectal carcinoma: To investigate the function of ADHFE1, we selected a DLD-1 among the CRC cell lines, without expression of ADHFE1. We transfected the DLD-1 with pcDNA3.1 vector containing the coding region of ADHFE1. To substantiate this finding, the level of differentiation marker, including ALP, CEA and Cdx2, were determined.