To investigate the function of ADHFE1, we selected a DLD-1 among the CRC cell lines, without expression of ADHFE1. We transfected the DLD-1 with pcDNA3.1 vector containing the coding region of ADHFE1. To substantiate this finding, the level of differentiation marker, including ALP, CEA and Cdx2, were determined. Here, CDX2 is linked to colorectal carcinoma.