To confirm the methylation-specific PCR results in CRC cell lines and tissues, we selected 6 paired CRC and adjacent normal colorectal mucosa, and 3 CRC cell lines, such as WiDr, HT 116 and HT29, which had shown hypermethylation of ADHFE1 promoter in methylation-specific PCR. This evidence concerns the gene ADHFE1 and colorectal carcinoma.