In order to determine the impact of NURR1 silencing on breast cancer cell proliferation, we stably transfected MDA-MB-468 and MDA-MB-231 cells with a short hairpin RNA (shRNA) encoding plasmids directed toward either NURR1 or a scrambled hairpin RNA as a control, (4A2KD- and Vec-, respectively), resulting in suppression of NURR1 protein expression in 4A2KD-468 and 4A2KD-231 cells. The gene discussed is NR4A2; the disease is breast cancer.