Annexin V/PI staining (Figure 6B), Caspase 3/7 activity assay (Figure 6C), and TUNEL assay (Figure 6D) in suspended hepatoma cells showed that Klotho-induced anoikis resistance was reversed by inactivating PAK1 kinase activity with PAK1 K299R cotransfection or IPA3 treatment. This evidence concerns the gene PAK1 and hepatocellular carcinoma.