To further identify PAK1 activation as downstream effector of VEGFR2 signal in Klotho-mediated anoikis resistance, functional alterations were analyzed after PAK1 activation with constitutive active mutant PAK1 T423E cotransfection in the presence of VEGFR2 inhibition with VEGR2 inhibitor Axitinib or its blocking antibody treatment in Klotho-overexpressed hepatoma cells. The gene discussed is KL; the disease is hepatocellular carcinoma.