Taken the percentage of positive cancer cells on each slide ranging from 10% to 90% into account, the cutoff value of Klotho staining was set on the median H-score at 1.5 and 52 HCC patients were divided into high Klotho expression group (H-score>1.5) with 33 (63.46%) cases and low Klotho expression group (H-score≤1.5) with 19 (36.54%) cases (Table 1). Here, KL is linked to cancer.