As shown in Table 1, Klotho overexpression strongly correlated with liver cirrhosis (P = 0.023), tumor multiplicity (P = 0.007), and venous invasion (P = 0.021) of HCC patients, whereas it was not associated with age, gender, HBsAg, tumor size, microsatellite nodules, tumor encapsulation, intrahepatic metastasis,tumor stage, or BCLC stage. This evidence concerns the gene KL and neoplasm.