As shown in Table 1, Klotho overexpression strongly correlated with liver cirrhosis (P = 0.023), tumor multiplicity (P = 0.007), and venous invasion (P = 0.021) of HCC patients, whereas it was not associated with age, gender, HBsAg, tumor size, microsatellite nodules, tumor encapsulation, intrahepatic metastasis,tumor stage, or BCLC stage. The gene discussed is KL; the disease is cirrhosis of liver.