We counted IHC ribbons from 3–5 animals per group (unexposed, no-tinnitus, tinnitus) as a correlate of the number of afferent auditory fibers [24], [37] using antibodies directed against CtBP2/RIBEYE in combination with a postsynaptic marker, the glutamate receptor isoform 4 (GluR4) [24] (Fig. 1E and F). The gene discussed is CTBP2; the disease is Tinnitus.