PRNP and Creutzfeldt Jacob disease: As expected, by probing with 3F4 antibody, two major sets of PrP spots were observed in the deglycosylated PrP from non-CJD: PrP 2D spots III migrating at 27-29 kDa with pI 7.0-9.6 corresponding to full-length PrP, and PrP 2D spots VI migrating at ~19-22 kDa with pI 6.1-8.1 corresponding to the N-terminally truncated PrP (Fig. 7D).