In the samples from non-CJD and sCJD, only one PrP band was detected in supernatants (Fig. 5A, S2) and pellets (Fig. 5B, P2), migrating at ~28-29 kDa, which corresponds to the full-length deglycosylated PrPWt. Here, PRNP is linked to Creutzfeldt Jacob disease.