In contrast, in the samples from fCJDIns, besides the ~28-29 kDa PrPWt band seen in non-CJD and sCJD samples, an additional PrP band migrating at ~33-34 kDa was detected in supernatants (Fig. 5A, S2) and pellets (Fig. 5B, P2), which corresponds to the full-length PrPIns with six octapeptide repeats containing 48 extra residues. Here, PRNP is linked to Creutzfeldt Jacob disease.