In this study, the Ad5/F35-APE1 adenoviral siRNA vector, which was constructed in our laboratory, was used to enhance the killing effect of HpD-PDT on A549 cells in vitro and in vivo, which was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry, enzyme linked immunosorbent assay (ELISA) and western blot, to provide a theoretical reference for the future treatment of targeted-APE1 combined with HpD-PDT in nonsmall cell lung cancer. This evidence concerns the gene APEX1 and lung cancer.