To shed more light in the proliferative effects of miR-191/425 in ERα positive breast cancer cells, flow cytometric analyses of transiently-transfected cells were performed and revealed an increased number of cells in G1 and fewer cells in G2/M following knockdown of either miR-191 or miR-425 compared to control cells (Figure 3B and Figure S8A). Here, ESR1 is linked to breast carcinoma.