All together our experiments demonstrate a duality in the biological role of miR-191/425 cluster in breast cancer: estrogen dependent-high levels of miR-191/425 induce proliferation in ERalpha positive cells by suppressing a strong tumor-suppressor gene, such as EGR1; low levels of miR-191/425 cluster are essential for the high expression of important modulators, such as SATB1, CCND2 and FSCN1, which confer a proliferative advantage to aggressive breast cancer cells. Here, EGR1 is linked to breast cancer.