In some cases the appropriate choice of analysis is obvious when the specific cause of disease is known, i.e., astrocytes from an ALS model (mutant TDP-43) iPSC lines have an increased expression and mislocalization of TDP-43 protein (Serio et al., 2013), and astrocytes from Alzheimer's disease models (sporadic cases and mutant APP) have Aβ oligomer accumulation (Kondo et al., 2013). Here, TARDBP is linked to early-onset autosomal dominant Alzheimer disease.