Two endometrial cancer cell lines, HEC-1B containing relatively lower endogenous HE4 levels and Ark2 with relative higher endogenous HE4 levels (data not shown), were transfected with pcDNA 3.1-Myc-His-HE4 to achieve ectopic overexpression of HE4. The gene discussed is WFDC2; the disease is endometrial cancer.