In concordance with the findings of previous studies showing that TLR7 activation triggers production of proinflammatory cytokines [10,11,38], our results showed elevated levels of serum IL-1β, IL-6, IL-18, and IFN-α positively correlated with the expression levels of TLR7 signaling molecules in AOSD patients. This evidence concerns the gene TLR7 and adult-onset Still disease.