The correlations between the expression levels of MIF and CXCR4 in different cell subsets in tumor microenvironment and prognostic factors were assessed to determine the clinical relevance and predictive value of the MIF and CXCR4 expression in different cell subsets of tumor microenvironments of ESCC. This evidence concerns the gene CXCR4 and esophageal squamous cell carcinoma.