Furthermore, a multivariate COX ’s Proportional Hazard Model, in which tumor size, LN metastasis, histologic grade , vessel invasion, and TFPI-2 expression were included, showed that loss of TFPI-2 expression was an independent prognostic factor for DFS in breast cancer patients (Exp(B)=0.316, 95.0% CI 0.191~0.532, P <0.01). This evidence concerns the gene TFPI2 and breast cancer.