Thus far, the Sanger DNA sequencing method is the reference method used for the detection and identification of EGFR mutations in tumor cells, because it provides the exact nucleotide sequence of the segment amplified, despite its sensitivity is lower than others, especially in the case of small tumor samples, since it requires at least 50% of mutated tumor cells[10], corresponding to 20–25% of mutated DNA in an heterozygous case[8]. Here, EGFR is linked to neoplasm.