IFNG and infection: These results together with the high production of IgG2a subclass indicate a Th1 pattern, and were consistent with reports from Aparicio-Burgos et al.; the circulatory cytokine levels (IFN-γ and IL-10) were below the detection limits before and after immunization with a multi-component DNA-prime/DNA-boost vaccine TcVac1— vaccine constituted of antigen-encoding plasmids (pCDNA3.TcG1, pCDNA3.TcG2 and pCDNA3.TcG4) and IL-12- and GMCSF-expression plasmids—, and all the dogs responded to infection with increases in IFN-γ levels (580 pg/mL approximately at 15 days post infection) [27].