GFAP and scrapie: Prionopathy associated spongiosis, PrPSc deposition and gliosis (assessed by GFAP immunoreactivity) were evaluated semiquantitatively in the four analyzed brain regions (cSc, Mobl, Cc and Dien) from 6 controls, 4 preclinical and 7 clinical scrapie-infected sheep (Figure 1).