In order to know whether hypoxia status is related to EMT in HCC, we firstly investigated expression levels of HIF-1α, HIF-2α, SNAI1, Twist, E-cadherin, N-cadherin and Vimentin in a tissue array containing 66 HCC samples from human patients by immunohistochemistry. This evidence concerns the gene EPAS1 and hepatocellular carcinoma.