In order to clarify the association of the immunophenotypic patterns of NPM1-mutated AML with other gene mutations in AML, we checked class I (FLT3-ITD, FLT3-TKD, PTPN11, JAK2, KIT, NRAS, KRAS, and WT1) and class II gene mutations (CEBPA and MLL-PTD). Here, WT1 is linked to acute myeloid leukemia.