CAMK2A and Anxiety: Along these lines, Refojo et al. (2011) demonstrated that overexpression of limbic CRF in CRF-COECamk2aCre mice (Camk2aCre, Cre driven by the calcium/calmodulin-dependent protein kinase type II alpha chain promoter) resulted in increased anxiety-like behavior too, suggesting that limbic CRF in particular would be instrumental in mediating anxiety-like behavior.