In this study, a systemic comparison of three PSPs (a −650/+30 bp PSA, a −426/+28 bp PB, and a Cla I truncated 1.1 kb MMTV LTR) activity and specificity in the canine and mouse models followed by a PSP-driven E1-mediated oncolytic approach on prostate cancer has been conducted. Here, KLK3 is linked to prostate cancer.