Genotype association in additive model revealed that the risk genotypes (AGTR1 rs3772627TT, AGTR1 rs3772630AA and PNPLA3 rs738409GG) versus one risk factor (AGTR1 rs3772627TC, AGTR1 rs3772630AG and PNPLA3 rs738409CG) versus the protective genotypes (AGTR1 rs3772627CC, AGTR1 rs3772630GG and PNPLA3 rs738409CC), conferred enhanced risk for NAFLD (OR 2.23, 95% CI 1.16–4.31, p = 0.017). Here, PNPLA3 is linked to metabolic dysfunction-associated steatotic liver disease.