Stable transfection of the FaDu HNSCC tumor cell line (which has low endogenous Lin28b expression) with this plasmid led to a significant increase in Lin28b expression at both the mRNA and protein levels in two selected stable clones (A1 and D1), compared to GFP-transfected control cells (Fig 1A and B). The gene discussed is LIN28B; the disease is head and neck squamous cell carcinoma.