CALR and systemic lupus erythematosus: Anti-DFS70 antibodies have been reported in approximately 3% of SLE patients [14], but the detection of anti-DFS70 as detected by IIF may be problematic because these sera are usually accompanied by other antibodies such as anti-dsDNA, anti-SS-A/Ro, or anti-Sm, which may mask the DFS IIF staining pattern.