Among the four experimental cell lines (es-LNCaP, ls-LNCaP, scr-ls-LNCaP, and si-ls-LNCaP), we confirmed the expression level of five prostate cancer related proteins (AR, HSP27, CLU, GRP78, and c-FLIP) with immunocytochemical staining (Figure 2). The gene discussed is CLU; the disease is prostate cancer.