Similar analysis for brain cancer showed that activated TP53 was up-regulated in the cancer samples versus the normal controls (p = 3.40×10−9, two tailed t-test), whereas wild-type TP53 were down-regulated in the cancer samples versus the normal controls (p = 1.49×10−18, two tailed t-test; Figure 3B). The gene discussed is TP53; the disease is cancer.