Considering the relations described between (i) the C1-metabolism and PC synthesis, (ii) observed changes in PL contents and PC signatures upon HF diet feeding and (iii) the activation of PPARα by a diacyl-phosphatidylcholine identified as a natural PPARα ligand, the following questions arise: how does a high dietary fat load impact on the hepatic C1-metabolism pathways at the levels of gene and protein expression as well as metabolites? This evidence concerns the gene PPARA and hydrops fetalis.