CBS and hydrops fetalis: Analysis of hepatic DNA methylation in the CpG island regions P1 and P2 of the Cbs promoter in control and HF mice revealed no detectable or low CpG methylation of AciI and HpaII restriction sites (Cbs promoter HpaII methylation; 8.08±0.54% in control animals and 8.46±0.37% in animals on HF diet) whereas the AciI restriction site analysis of the Cbs intragenic region I7 showed CpG methylation of 61.68±4.25% in control liver genomic DNA and 65.24±3.95% in genomic DNA of HF diet liver samples.