NEFL and amyotrophic lateral sclerosis: In EOMs from both controls and ALS donors, axons visualized by NF-L staining showed contact with the muscle fibers through separate outspread branches in the synaptic area furling into a knot (i.e. the terminal nerve axons; Figure 1A), or were interwoven with postsynaptic labeling by α-BTx (Figure 1B).