In the present study, we used markers of the different elements of the NMJ: neurofilament (NF) and synaptophysin to study the nerve endings, S100B, p75 neurotrophin receptor (p75NTR) and glial fibrillary acidic protein (GFAP) to study the terminal Schwann cells, and α-bungarotoxin to detect the muscle side of the synapse, in order to further elucidate the differences in the impact of ALS on EOM vs limb NMJs. Here, NGFR is linked to amyotrophic lateral sclerosis.