Furthermore, to determine the potential mechanisms of Notch1 and Notch3 in the migration and invasion of HCC cells, we examined the effect of down-regulated Notch1 and Notch3 on metastasis-associated molecules, such as CD44v6, E-cadherin, MMP-2, MMP-9, and uPA. This evidence concerns the gene MMP9 and hepatocellular carcinoma.