To assess the effectiveness and tissue-specificity of the AFP-Cre/LoxP-shRNA system, AFP-producing HCC cells (HCCLM3, HepG2, and Hep3B), normal hepatocyte (L-02), and non-HCC cancer cells (Hela and SW1116) were infected with the AFP-Cre/LoxP-shRNA system at a MOI of 1,5,10 or 20. This evidence concerns the gene AFP and cancer.