In fact, our data for the first time demonstrate that, in AM, PKR signalling and subsequent transcriptional activity of NF-κB are indispensable for type I IFN release upon IV infection, suggesting a macrophage-specific way of signal transduction and adding to a previous study highlighting NF-κB as key regulator of the cellular type I IFN response towards a highly pathogenic IV [27]. This evidence concerns the gene EIF2AK2 and infection.