In this study, we focused on the downregulated miR-134, in human glioma tissues and in glioblastoma cell line U87 then compared it to normal brain tissues, finding that ectopic expression of miR-134 reduced the level of Nanog expression, causing inhibition of proliferation, invasiveness and migration, it also increased apoptosis in glioblastoma cells. The gene discussed is NANOG; the disease is central nervous system cancer.