In addition, we observed that the action of carbachol was significantly reduced by: the non selective muscarinic antagonist atropine (42±12%), by the M3 receptor antagonist 4-DAMP (53±3%) or by tropicamide (37±8%) an M4 selective antagonist, confirming the participation of these receptors in carbachol-induced VEGF-A production by tumor cells. The gene discussed is VEGFA; the disease is neoplasm.