Of note, almost all other published cell culture-based RNAi screens in GBM cell lines did not pick up RIOK1 or RIOK2 because these screens were not designed to distinguish between kinases that were required for genotypic-specific survival or growth of GBM cell lines, and instead focused on kinases that showed a common requirement in all glioblastoma cell lines tested, be they mutant or wild-type for p53, EGFR, or PTEN [67], [68], [69]. Here, EGFR is linked to glioblastoma.