To select a suitable cell model, we screened the expression of Osx in various cell lines including human embryonic kidney HEK 293T cells, human lung carcinoma A549 cells, human pancreatic adenocarcinoma SW1990 cells, human osteosarcoma MG-63 cells and Saos-2 cells by Western blot analysis. This evidence concerns the gene SP7 and osteosarcoma.