To delineate the effect of IR on NFκB expression and DNA-binding activity in hypoxic breast cancer cells and further to elucidate the efficacy of nutraceuticals in this setting, human BCa (MCF-7) cells cultured under hypoxic conditions were either mock-irradiated or exposed to IR (2 Gy) with or without EF24, NLE, CUR, GEN, RES and RSE pre-treatment and harvested after 24 h post-IR. This evidence concerns the gene NFKB1 and breast carcinoma.